Single-nucleotide polymorphism (SNPs) have become the genetic markers of choice in many ecological and evolutionary studies. To discover and genotype SNPs across the whole genome, especially for non-model organisms, is becoming a crucial genetic tool. Here at the Centre for Marine Evolutionary Biology of University of Gothenburg (https://cemeb.science.gu.se)I am learning a simple and flexible method for genome-wide genotyping – 2b-RAD for detecting SNPs, that uses type IIB restriction enzymes. These enzymes cleave genomic DNA upstream and downstream of the target site, producing tags of uniform length that are ideally suited for sequencing on existing next-generation platforms. These sequences are useful for linkage mapping and profiling genetic variation in natural populations.
APRIL – OCTOBER 2018